Label free highly multimodal nonlinear endoscope using hollow core fiber (Conference Presentation)

Hervé Rigneault; Dylan Septier; Gaelle Brevalle-Wasilewski; Naveen Gajendra Kumar; Vasyl Mytskaniuk; Remi Habert; Damien Labat; Karen Baudelle; Alexandre Cassez; Geraud Bouwmans; Alexandre Kudlinski

doi:10.1117/12.2648085

15 March 2023 Label free highly multimodal nonlinear endoscope using hollow core fiber (Conference Presentation)

Hervé Rigneault, Dylan Septier, Gaelle Brevalle-Wasilewski, Naveen Gajendra Kumar, Vasyl Mytskaniuk, Remi Habert, Damien Labat, Karen Baudelle, Alexandre Cassez, Geraud Bouwmans, Alexandre Kudlinski

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840T (2023) https://doi.org/10.1117/12.2648085
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We demonstrate a highly multimodal nonlinear micro-endoscope for real-time, label-free imaging of biological tissues. The endoscope can perform two and three photon excited fluorescence, second, third harmonic and CARS imaging for different excitation wavelengths. Ultrashort pulses are delivered to the sample by a double-clad antiresonant hollow core fiber over the 800-1800 nm spectral band. The fiber tip is placed into a doubly resonant piezoelectric tube which allows a spiral scanning on the sample. The endoscope distal head containing the scanning device and the GRIN micro-objective is 1.5 mm in diameter and 35 mm long. Real-time nonlinear imaging at 10 frame/s is demonstrated.

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Hervé Rigneault, Dylan Septier, Gaelle Brevalle-Wasilewski, Naveen Gajendra Kumar, Vasyl Mytskaniuk, Remi Habert, Damien Labat, Karen Baudelle, Alexandre Cassez, Geraud Bouwmans, and Alexandre Kudlinski "Label free highly multimodal nonlinear endoscope using hollow core fiber (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840T (15 March 2023); https://doi.org/10.1117/12.2648085

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KEYWORDS

Endoscopes

GRIN lenses

Luminescence

Real time imaging

Tissues

Nonlinear optics

Polymers

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