Event-based sensor for fast and dense single-molecule localization microscopy (Conference Presentation)

Clément Cabriel; Christian Specht; Ignacio Izeddin

doi:10.1117/12.2648971

15 March 2023 Event-based sensor for fast and dense single-molecule localization microscopy (Conference Presentation)

Clément Cabriel, Christian Specht, Ignacio Izeddin

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC123860G (2023) https://doi.org/10.1117/12.2648971
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In this contribution, we demonstrate the feasibility of fluorescence single-molecule localization microscopy (SMLM) imaging with an event-based (or neuromorphic vision) sensor. Event-based sensors are matrices of independent pixels sensitive to intensity variations only, with a microsecond range temporal resolution. We have integrated such an event-based sensor in a typical SMLM set-up and characterized its localization precision and response linearity. We obtain super-resolution images of biological samples of the same resolution than with conventional scientific cameras. Finally, we exploit the unique characteristics of event-based SMLM to perform high-density acquisitions with overlapping PSFs, achieving much better results than with frame-based cameras.

Conference Presentation

Citation Download Citation

Clément Cabriel, Christian Specht, and Ignacio Izeddin "Event-based sensor for fast and dense single-molecule localization microscopy (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC123860G (15 March 2023); https://doi.org/10.1117/12.2648971

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