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15 March 2023 Label-free and live-cell Raman and autofluorescence imaging of drug distribution and induced cytotoxicity (Conference Presentation)
Menglu Li, Hao-Xiang Liao, Kazuki Bando, Yasunori Nawa, Satoshi Fujita, Katsumasa Fujita
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920C (2023) https://doi.org/10.1117/12.2649911
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Simultaneous observation of cytotoxicity and drug distribution at the effector site will bring new insights into understanding mechanism of action of drug. Here, we demonstrated the application of Raman microscopy to visualize drug-induced cytotoxicity and drug distribution as well as its mechanism of action at a real-time and single-cell level. The drug concentration and treatment-dependent decrease of reduced cyt c was found as an early marker of cell apoptosis which is more sensitive than the conventional cell viability assay. This method allows for us to screen the drug cytotoxicity and approach the mechanism at the same time, thereby reducing the cost and time of drug screening.
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Menglu Li, Hao-Xiang Liao, Kazuki Bando, Yasunori Nawa, Satoshi Fujita, and Katsumasa Fujita "Label-free and live-cell Raman and autofluorescence imaging of drug distribution and induced cytotoxicity (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920C (15 March 2023); https://doi.org/10.1117/12.2649911
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KEYWORDS
Raman spectroscopy
Auto-fluorescence imaging
Microscopy
Visualization
Cell death
Chemical analysis
Luminescence
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