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15 March 2023 Quantitative chemical imaging of live cells using advanced IR microscopy (Conference Presentation)
Yow-Ren Chang, Seong-Min Kim, Young Jong Lee
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920T (2023) https://doi.org/10.1117/12.2649391
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Infrared (IR) absorption microscopy is a sensitive label-free chemical imaging tool for biomolecules in cells and tissues. However, quantitative measurements in hydrated biological samples remain a challenge due to the strong water absorption in the fingerprint region, in particular near 1650 cm-1, which critically overlaps with the protein amide I peak. Here, we present a new approach to overcome the challenge by using a Solvent Absorption Compensation (SAC) unit, which dynamically adjusts the intensity of incident light to compensate for the strong water absorption. We demonstrate the capability to perform quantitative absorption imaging of proteins (amide I) in live fibroblast cells in a 25 µm thick layer of water. This work enables future label-free, quantitative chemical imaging of biomolecules in the living cell.
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Yow-Ren Chang, Seong-Min Kim, and Young Jong Lee "Quantitative chemical imaging of live cells using advanced IR microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920T (15 March 2023); https://doi.org/10.1117/12.2649391
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KEYWORDS
Infrared imaging
Imaging spectroscopy
Infrared microscopy
Microscopy
Absorption
Proteins
FT-IR spectroscopy
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