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We have developed a software platform to provide real-time readout of neuronal activity and downstream analysis which enables informed real-time modulation of neuronal activity through photostimulation during image acquisition.
The downstream analysis within the real-time loop is rapid enough (at 30Hz for 250 neurons) to operate at widely used image acquisition rates.
Wolfgang Losert andDulara de Zoysa
"Real time spatial light modulation for informed photostimulation of neuronal cells", Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC126491B (5 October 2023); https://doi.org/10.1117/12.2682243
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Wolfgang Losert, Dulara de Zoysa, "Real time spatial light modulation for informed photostimulation of neuronal cells," Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC126491B (5 October 2023); https://doi.org/10.1117/12.2682243