Deep ultraviolet transmittance and scattering microscopy for virtual histology

Brendyn D. Cikaluk; Mohammad H. Masoumi; Brendon S. Restall; Matthew T. Martell; Nathaniel J. M. Haven; Roger J. Zemp

doi:10.1117/12.3001806

13 March 2024 Deep ultraviolet transmittance and scattering microscopy for virtual histology

Brendyn D. Cikaluk, Mohammad H. Masoumi, Brendon S. Restall, Matthew T. Martell, Nathaniel J. M. Haven, Roger J. Zemp

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540P (2024) https://doi.org/10.1117/12.3001806
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate a rapid deep ultraviolet transmission and scattering microscopy system which utilizes a single 266 nm nanosecond pulsed laser source to generate virtual histology images. By detecting both the transmitted and back-scattered 266 nm radiation, distinct contrasts of the tissue section are produced. Maximally realistic virtual histology images are then generated using each contrast as separate inputs to a CycleGAN deep neural network. This work demonstrates virtual histology images of mm-scale areas at fine resolutions (< 500 nm) within minutes. This system greatly simplifies the optics and alignment required for imaging, and could provide future utility for clinical studies.

Conference Presentation

Citation Download Citation

Brendyn D. Cikaluk, Mohammad H. Masoumi, Brendon S. Restall, Matthew T. Martell, Nathaniel J. M. Haven, and Roger J. Zemp "Deep ultraviolet transmittance and scattering microscopy for virtual histology", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540P (13 March 2024); https://doi.org/10.1117/12.3001806

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