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13 March 2024 Picosecond hyperspectral stimulated Raman scattering microscopy below 400 wavenumbers
Chi Zhang, Matthew Clark, Bin Dong
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550A (2024) https://doi.org/10.1117/12.3009819
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The low-frequency Raman region below 400 wavenumbers has rich chemical information to identify elements and compounds with low vibrational energies. Such low-frequency vibrational modes are usually probed by spontaneous Raman scattering which has low acquisition speed or femtosecond impulsive Raman scattering that requires ultrashort laser pulse and interferometry. High-speed hyperspectral stimulated Raman scattering (hSRS) microscopy has been developed based on spectral focusing but cannot detect low-frequency vibrational transitions. We extended the frequency range of picosecond hSRS microscopy to measure vibrational transitions below 400 cm-1. Using spectral focusing, we can perform hSRS imaging for a ~200 cm-1 spectral window tunable from 200-4000 cm-1.
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Chi Zhang, Matthew Clark, and Bin Dong "Picosecond hyperspectral stimulated Raman scattering microscopy below 400 wavenumbers", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550A (13 March 2024); https://doi.org/10.1117/12.3009819
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KEYWORDS
Microscopy
Picosecond phenomena
Raman scattering
Raman spectroscopy
Vibration
Biological imaging
Femtosecond phenomena
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