Differentiation of highly metastatic nasopharyngeal carcinoma cells using multiphoton microscopy

Zhenlin Zhan; Zhenzhen Sun; Jingwen Li; Qing Ye; Shuangmu Zhuo; Shusen Xie

doi:10.1117/12.2245983

31 October 2016 Differentiation of highly metastatic nasopharyngeal carcinoma cells using multiphoton microscopy

Zhenlin Zhan, Zhenzhen Sun, Jingwen Li, Qing Ye, Shuangmu Zhuo, Shusen Xie

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002430 (2016) https://doi.org/10.1117/12.2245983
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

The primary hypothesis tested in the study was that nasopharyngeal carcinoma (NPC) cells at different stage of invasion and metastasis can be differentiated using multiphoton microscopy (MPM). CNE1 and CNE2Z cells were cultured and used in this study. The activity of cell migration and invasion was measured using Transwell assays. At the same time, the morphologic features were quantified from the multiphoton images. The measurements of Transwell migration and invasion showed that the invasion and migration of CNE2Z cells were significantly enhanced when compared with that of CNE1 cells. Also, statistically significant differences in the morphologic features were found between two kinds of cancer cells. In conclusion, it is feasible to use MPM to differentiate cancer cells with different stage of invasion and metastasis.

Citation Download Citation

Zhenlin Zhan, Zhenzhen Sun, Jingwen Li, Qing Ye, Shuangmu Zhuo, and Shusen Xie "Differentiation of highly metastatic nasopharyngeal carcinoma cells using multiphoton microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002430 (31 October 2016); https://doi.org/10.1117/12.2245983

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