Visualization of active ingredients uptake in seed coats with stimulated Raman scattering microscopy

Chun-Chin Wang; Fei-Yian Yoong; Steven Penfield; Julian Moger

doi:10.1117/12.2252080

21 February 2017 Visualization of active ingredients uptake in seed coats with stimulated Raman scattering microscopy

Chun-Chin Wang, Fei-Yian Yoong, Steven Penfield, Julian Moger

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 1006928 (2017) https://doi.org/10.1117/12.2252080
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The tissues surrounding the seeds play an important role in the control of germination vigour and in the uptake of active ingredients (AIs) applied as seed dressings. The seeds can reduce costs through more efficient germination rates and have less environmental impact due to more efficient use of AIs. In this study, we use epi-detected stimulated Raman scattering (SRS) microscopy with a fiber laser source for analyzing modes of D₂O into intact seeds and determining uptake kinetics. We demonstrate that SRS microscopy is an ideal tool for visualizing uptake of AIs and worthy of further applications in real field conditions.

Citation Download Citation

Chun-Chin Wang, Fei-Yian Yoong, Steven Penfield, and Julian Moger "Visualization of active ingredients uptake in seed coats with stimulated Raman scattering microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006928 (21 February 2017); https://doi.org/10.1117/12.2252080

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