New sensorless wavefront estimation approach for two-photon scanning microscopy

J. Teixeira; D. Champelovier; J-M. Conan; L. Mugnier; S. Meimon; N. Balla; S. Reichinnek; T. Tressard; R. Cossart; S. Monneret; H. Rigneault; A. Malvache

doi:10.1117/12.2250323

21 February 2017 New sensorless wavefront estimation approach for two-photon scanning microscopy

J. Teixeira, D. Champelovier, J-M. Conan, L. Mugnier, S. Meimon, N. Balla, S. Reichinnek, T. Tressard, R. Cossart, S. Monneret, H. Rigneault, A. Malvache

Author Affiliations +

Proceedings Volume 10073, Adaptive Optics and Wavefront Control for Biological Systems III; 1007317 (2017) https://doi.org/10.1117/12.2250323
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The landscape of biomedical research in neuroscience has changed dramatically in recent years as a result of spectacular progress in dynamic microscopy. In this context Adaptive Optics allows in-depth imaging by correcting aberrations induced by the biological sample, the key issue being then the ability to perform an accurate and reliable wavefront sensing (WFS). We present here a limitation of modal sensorless WFS in the case of a heterogeneous medium. We then build a new method called Axially-Locked Modal Sensorless (ALMS) that exploits these heterogeneities to overcome this limitation. The new method is simulated and compared to standard modal sensorless. The simulation results show a more accurate wavefront estimation even in the case of a strongly aberrated biological media.

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J. Teixeira, D. Champelovier, J-M. Conan, L. Mugnier, S. Meimon, N. Balla, S. Reichinnek, T. Tressard, R. Cossart, S. Monneret, H. Rigneault, and A. Malvache "New sensorless wavefront estimation approach for two-photon scanning microscopy", Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 1007317 (21 February 2017); https://doi.org/10.1117/12.2250323

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