Wavefront sensorless adaptive optics versus sensor-based adaptive optics for in vivo fluorescence retinal imaging (Conference Presentation)

Daniel J. Wahl; Pengfei Zhang; Yifan Jian; Stefano Bonora; Marinko V. Sarunic; Robert J. Zawadzki

doi:10.1117/12.2253368

24 April 2017 Wavefront sensorless adaptive optics versus sensor-based adaptive optics for in vivo fluorescence retinal imaging (Conference Presentation)

Daniel J. Wahl, Pengfei Zhang, Yifan Jian, Stefano Bonora, Marinko V. Sarunic, Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 10073, Adaptive Optics and Wavefront Control for Biological Systems III; 100731G (2017) https://doi.org/10.1117/12.2253368
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Adaptive optics (AO) is essential for achieving diffraction limited resolution in large numerical aperture (NA) in-vivo retinal imaging in small animals. Cellular-resolution in-vivo imaging of fluorescently labeled cells is highly desirable for studying pathophysiology in animal models of retina diseases in pre-clinical vision research. Currently, wavefront sensor-based (WFS-based) AO is widely used for retinal imaging and has demonstrated great success. However, the performance can be limited by several factors including common path errors, wavefront reconstruction errors and an ill-defined reference plane on the retina. Wavefront sensorless (WFS-less) AO has the advantage of avoiding these issues at the cost of algorithmic execution time. We have investigated WFS-less AO on a fluorescence scanning laser ophthalmoscopy (fSLO) system that was originally designed for WFS-based AO. The WFS-based AO uses a Shack-Hartmann WFS and a continuous surface deformable mirror in a closed-loop control system to measure and correct for aberrations induced by the mouse eye. The WFS-less AO performs an open-loop modal optimization with an image quality metric. After WFS-less AO aberration correction, the WFS was used as a control of the closed-loop WFS-less AO operation. We can easily switch between WFS-based and WFS-less control of the deformable mirror multiple times within an imaging session for the same mouse. This allows for a direct comparison between these two types of AO correction for fSLO. Our results demonstrate volumetric AO-fSLO imaging of mouse retinal cells labeled with GFP. Most significantly, we have analyzed and compared the aberration correction results for WFS-based and WFS-less AO imaging.

Conference Presentation

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Daniel J. Wahl, Pengfei Zhang, Yifan Jian, Stefano Bonora, Marinko V. Sarunic, and Robert J. Zawadzki "Wavefront sensorless adaptive optics versus sensor-based adaptive optics for in vivo fluorescence retinal imaging (Conference Presentation)", Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 100731G (24 April 2017); https://doi.org/10.1117/12.2253368

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KEYWORDS

Adaptive optics

Sensors

Retinal scanning

Wavefronts

In vivo imaging

Luminescence

Aberration correction

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