Nanosensors for nucleic acid targets detection using SERS

Pietro Strobbia; Bridget M. Crawford; Hsin-Neng Wang; Tuan Vo-Dinh

doi:10.1117/12.2267757

3 May 2017 Nanosensors for nucleic acid targets detection using SERS

Pietro Strobbia, Bridget M. Crawford, Hsin-Neng Wang, Tuan Vo-Dinh

Proceedings Volume 10215, Advanced Environmental, Chemical, and Biological Sensing Technologies XIV; 1021508 (2017) https://doi.org/10.1117/12.2267757
Event: SPIE Commercial + Scientific Sensing and Imaging, 2017, Anaheim, CA, United States

Abstract

Single cell analysis can aid the study of molecular events responsible for cellular functions and unveil their connections to the biological functions of an organism. Biosensors based on surface enhanced Raman spectroscopy (SERS) can be used to this end and offer several advantages over other sensing platforms, such as sensitivity and multiplexed capabilities, among others. While SERS nanosensors/nanoparticles have been used for analysis in single cells, the delivery of such biosensors relies on cellular uptake, which requires long incubation time and has different efficiencies among cell lines. Nanosensors based on tapered optical fibers, instead, can be inserted in single cells and detect target molecules in subcellular compartment. The combination of these sensing devices with the transduction mechanism of nucleic acid based nanoprobes (i.e. inverse molecular sentinels) will permit the more direct detection of nucleic acids within single cells. This paper presents the development of tapered fiber-based biosensors for the detection of nucleic acid biomarkers in plant cells. The use of inverse molecular sentinels in plant cell was demonstrated. Sensors based on tapered fibers were fabricated and used to measure SERS from a single cell.

Conference Presentation

Citation Download Citation

Pietro Strobbia, Bridget M. Crawford, Hsin-Neng Wang, and Tuan Vo-Dinh "Nanosensors for nucleic acid targets detection using SERS", Proc. SPIE 10215, Advanced Environmental, Chemical, and Biological Sensing Technologies XIV, 1021508 (3 May 2017); https://doi.org/10.1117/12.2267757

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available