Simultaneous three-dimensional imaging of multi-focal microscopy

Chen Yen Lin; Yi-Tun Lin; Yuan Luo

doi:10.1117/12.2269600

18 April 2017 Simultaneous three-dimensional imaging of multi-focal microscopy

Chen Yen Lin, Yi-Tun Lin, Yuan Luo

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510Y (2017) https://doi.org/10.1117/12.2269600
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

High-speed microscopy three-dimensional (3D) microscopy based on trans-illumination is implemented with an amplitude light modulator placed at the Fourier plane of the system. The phase of an incident wave-front is modified and encoded with a defocus parameter to divert the light onto different portion of an image plane depending on their diffraction order and depth positions. The design of the grating pattern for the light modulated is discussed through the simulation and the experiment. 3D imaging capability is demonstrated through the experiment.

Citation Download Citation

Chen Yen Lin, Yi-Tun Lin, and Yuan Luo "Simultaneous three-dimensional imaging of multi-focal microscopy", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510Y (18 April 2017); https://doi.org/10.1117/12.2269600

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