Bayesian based fluorescence coded imaging using quantum dots

Takahiro Nishimura; Hitoshi Kimura; Yusuke Ogura; Jun Tanida

doi:10.1117/12.2269432

18 April 2017 Bayesian based fluorescence coded imaging using quantum dots

Takahiro Nishimura, Hitoshi Kimura, Yusuke Ogura, Jun Tanida

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510Z (2017) https://doi.org/10.1117/12.2269432
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Single-molecule localization techniques are effective to resolve fluorescence images with higher resolution. To increase the frame rate, high-density positions of individual fluorescence emitters should be measured. We are studying a Bayesian-based localization method for measuring high density molecular positions with fluorescence coded images. In this paper, a scheme of several color quantum dots aligned with DNA nanostructures are considered. We confirmed that the proposed method could be applied to fluorescence images of quantum dots experimentally and that the positions of the aligned fluorescence emitters at intervals of 80 nm could be measured with little errors in numerical simulations.

Citation Download Citation

Takahiro Nishimura, Hitoshi Kimura, Yusuke Ogura, and Jun Tanida "Bayesian based fluorescence coded imaging using quantum dots", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510Z (18 April 2017); https://doi.org/10.1117/12.2269432

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