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During long-term imaging, cells move out of the field of view. We have generated functionalized substrates containing rectangular areas, which were capable in keeping cells over the whole observation period.
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Sarah Mues, Inga Lilge, Holger Schönherr, Björn Kemper, Jürgen Schnekenburger, "Micro patterned surfaces allow long-term digital holographic microscopy live cell imaging," Proc. SPIE 10414, Advances in Microscopic Imaging, 104140S (28 July 2017); https://doi.org/10.1117/12.2287270