Fluorescence lifetime imaging and its applications in cellular microenvironment measurement and auxiliary diagnosis

Teng Luo; Svitlana M. Levchenko; Artem Pliss; Xiao Peng; Wei Yan; Paras N. Prasad; Liwei Liu; Junle Qu

doi:10.1117/12.2289017

19 February 2018 Fluorescence lifetime imaging and its applications in cellular microenvironment measurement and auxiliary diagnosis

Teng Luo, Svitlana M. Levchenko, Artem Pliss, Xiao Peng, Wei Yan, Paras N. Prasad, Liwei Liu, Junle Qu

Author Affiliations +

Proceedings Volume 10495, Biophotonics and Immune Responses XIII; 104950I (2018) https://doi.org/10.1117/12.2289017
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We present our recent work on the applications of fluorescence lifetime imaging microscopy(FLIM), including the monitoring of macromolecule dynamic changes in the nucleolar compartments and the auxiliary diagnosis of H and E-stained sections. We demonstrated the capability of FLIM to measure protein concentration in the specific cellular compartments in live cells. We proposed to use FLIM to monitor changes in intracellular protein concentration caused by various factors e.g. cell cycle progression, drug treatment etc. In the future, FLIM technology is expected to be combined with super-resolution optical imaging. FLIM with molecular resolution will have the potential to serve as a powerful tool for discovering new phenomena and revealing new mechanisms in biomedical research, which will effectively promote the development of life science.

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Teng Luo, Svitlana M. Levchenko, Artem Pliss, Xiao Peng, Wei Yan, Paras N. Prasad, Liwei Liu, and Junle Qu "Fluorescence lifetime imaging and its applications in cellular microenvironment measurement and auxiliary diagnosis", Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 104950I (19 February 2018); https://doi.org/10.1117/12.2289017

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KEYWORDS

Luminescence

Fluorescence lifetime imaging

Proteins

Refractive index

Signal attenuation

Tissues

Green fluorescent protein

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