Low-cost polarization microscopy for cholesterol crystals

Kyungmin Kim; Seonghee Cho; Taehoon Kim; Hyoeun Park; Jinmoo Kim; Seunghoon Lee; Yeonsu Kang; Kiyuk Chang; Chulhong Kim

doi:10.1117/12.2292855

20 February 2018 Low-cost polarization microscopy for cholesterol crystals

Kyungmin Kim, Seonghee Cho, Taehoon Kim, Hyoeun Park, Jinmoo Kim, Seunghoon Lee, Yeonsu Kang, Kiyuk Chang, Chulhong Kim

Author Affiliations +

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104971O (2018) https://doi.org/10.1117/12.2292855
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Because cholesterol crystals (Chcs) are a major cause of atherosclerosis, imaging Chcs in tissues with high sensitivity and specificity is important in diagnosing and predicting atherosclerosis. Polarizing microscopy (PM) has been widely used to image crystalline materials in tissues, but it has been difficult to distinguish Chcs from other crystalline materials in tissues. Thus, various methods such as fluorescent dye staining, Raman spectroscopy, and two-photon microscopy (TPM) have been developed to image Chcs with high sensitivity and specificity. However, these methods require expensive equipment or complex processes. Therefore, we have developed a low-cost, easy-to-use PM system using an LED light source that can distinguish Chcs from other crystalline materials with high sensitivity and specificity. Due to the nature of the LED spectrum in our system, collagen is displayed in yellow and Chcs in blue. In addition, we have improved the sensitivity and specificity by creating an aqueous condition on the sample. In the aqueous state, signals of yellowish collagen fibers were reduced and signals of Chcs were highlighted. The Chcs detection capability of our system was verified compared with the TPM image. In addition, clinical feasibility was shown by comparison with existing histological methods.

Citation Download Citation

Kyungmin Kim, Seonghee Cho, Taehoon Kim, Hyoeun Park, Jinmoo Kim, Seunghoon Lee, Yeonsu Kang, Kiyuk Chang, and Chulhong Kim "Low-cost polarization microscopy for cholesterol crystals", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971O (20 February 2018); https://doi.org/10.1117/12.2292855

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