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14 March 2018 An add-on adaptive optical module for laser scanning microscopy (Conference Presentation)
Ichun Anderson Chen, Wenzhi Sun, Yajie Liang, Daniel Milkie, Thomas Bifano, Na Ji
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Proceedings Volume 10502, Adaptive Optics and Wavefront Control for Biological Systems IV; 105020F (2018) https://doi.org/10.1117/12.2292944
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Wavefront-shaping devices incorporated into optical microscopy systems are capable of correcting sample-induced aberrations and recovering diffraction-limited imaging performance. The widespread dissemination and application of adaptive optical techniques, however, requires easy integration of adaptive optical modules, both in terms of hardware and software, into existing microscopes. We built an adaptive optical module with reduced complexity and simplified integration by utilizing a novel segmented deformable mirror and a standalone control software program. We demonstrated its ability to improve image brightness and resolution at depth in the mouse, zebrafish, and fly brains in vivo.
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Ichun Anderson Chen, Wenzhi Sun, Yajie Liang, Daniel Milkie, Thomas Bifano, and Na Ji "An add-on adaptive optical module for laser scanning microscopy (Conference Presentation)", Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105020F (14 March 2018); https://doi.org/10.1117/12.2292944
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KEYWORDS
Adaptive optics
Deformable mirrors
Integrated optics
Imaging systems
In vivo imaging
Microscopy
Optical microscopy
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