Phase from defocus

Ondrej Mandula; Cédric Allier; Lionel Hervé; Eric Denarier; Anne Fourest-Lieuvin; Sylvie Gory-Fauré; Angélique Vinit; Sophie Morales

doi:10.1117/12.2287693

23 February 2018 Phase from defocus

Ondrej Mandula, Cédric Allier, Lionel Hervé, Eric Denarier, Anne Fourest-Lieuvin, Sylvie Gory-Fauré, Angélique Vinit, Sophie Morales

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Proceedings Volume 10503, Quantitative Phase Imaging IV; 105031Q (2018) https://doi.org/10.1117/12.2287693
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We present a simple and compact phase imaging microscope for long-term observation of non-absorbing biological samples such as unstained cells in nutritive media. The phase image is obtained from a single defocused image taken with a standard wide-field microscope. Using a semi-coherent light source allows us to computationally re-focus image post-acquisition and recover both phase and transmission of the complex specimen. The simplicity of the system reduces both the cost and its physical size and allows a long-term observation of samples directly in a standard biological incubator. The low cost of the system can contribute to the democratization of science by allowing to perform complex long-term biological experiments to the laboratories with constrained budget. In this proceeding we present several results taken with our prototype and discuss the possibilities and limitations of our system.

Citation Download Citation

Ondrej Mandula, Cédric Allier, Lionel Hervé, Eric Denarier, Anne Fourest-Lieuvin, Sylvie Gory-Fauré, Angélique Vinit, and Sophie Morales "Phase from defocus", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031Q (23 February 2018); https://doi.org/10.1117/12.2287693

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