Ultra-sensitive fluorescent imaging-biosensing using biological photonic crystals

Kenny Squire; Xianming Kong; Bo Wu; Gregory Rorrer; Alan X. Wang

doi:10.1117/12.2292289

20 February 2018 Ultra-sensitive fluorescent imaging-biosensing using biological photonic crystals

Kenny Squire, Xianming Kong, Bo Wu, Gregory Rorrer, Alan X. Wang

Proceedings Volume 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV; 105060K (2018) https://doi.org/10.1117/12.2292289
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Optical biosensing is a growing area of research known for its low limits of detection. Among optical sensing techniques, fluorescence detection is among the most established and prevalent. Fluorescence imaging is an optical biosensing modality that exploits the sensitivity of fluorescence in an easy-to-use process. Fluorescence imaging allows a user to place a sample on a sensor and use an imager, such as a camera, to collect the results. The image can then be processed to determine the presence of the analyte. Fluorescence imaging is appealing because it can be performed with as little as a light source, a camera and a data processor thus being ideal for nontrained personnel without any expensive equipment. Fluorescence imaging sensors generally employ an immunoassay procedure to selectively trap analytes such as antigens or antibodies. When the analyte is present, the sensor fluoresces thus transducing the chemical reaction into an optical signal capable of imaging. Enhancement of this fluorescence leads to an enhancement in the detection capabilities of the sensor. Diatoms are unicellular algae with a biosilica shell called a frustule. The frustule is porous with periodic nanopores making them biological photonic crystals. Additionally, the porous nature of the frustule allows for large surface area capable of multiple analyte binding sites. In this paper, we fabricate a diatom based ultra-sensitive fluorescence imaging biosensor capable of detecting the antibody mouse immunoglobulin down to a concentration of 1 nM. The measured signal has an enhancement of 6× when compared to sensors fabricated without diatoms.

Conference Presentation

Citation Download Citation

Kenny Squire, Xianming Kong, Bo Wu, Gregory Rorrer, and Alan X. Wang "Ultra-sensitive fluorescent imaging-biosensing using biological photonic crystals", Proc. SPIE 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, 105060K (20 February 2018); https://doi.org/10.1117/12.2292289

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available