Improvement of the multiphoton fluorescence microscopy images quality using digital filtration

Y. V. Kistenev; V. V. Nikolaev; A. K. Drozdova; E. E. Ilyasova; E. A. Sandykova

doi:10.1117/12.2303643

16 April 2018 Improvement of the multiphoton fluorescence microscopy images quality using digital filtration

Y. V. Kistenev, V. V. Nikolaev, A. K. Drozdova, E. E. Ilyasova, E. A. Sandykova

Proceedings Volume 10614, International Conference on Atomic and Molecular Pulsed Lasers XIII; 106141Q (2018) https://doi.org/10.1117/12.2303643
Event: XIII International Conference on Atomic and Molecular Pulsed Lasers, 2017, Tomsk, Russia

Abstract

In our study we used rank-order filter, the emissions filter on the base of the criteria of Pearson, Gaussian filter and median filterfor improving the is fluorescence lifetime imaging microscopy (FLIM) data. The data obtained with the FLIM technology are the distribution with a pronounced peak, while during measurement the peak value is measured with an error. According to the analysisthe Gaussian filter is more useful to improve quality of FLIM data.Spatial filtering allows to reduce the noise component, obtained in the course of measurements, including reduction the influence of the individual bursts. Filtering in time scale allows to determine a peak value of intensity more accurately.This research was carried out using the equipment of Tomsk Regional Common Use Center of Tomsk State University.

Citation Download Citation

Y. V. Kistenev, V. V. Nikolaev, A. K. Drozdova, E. E. Ilyasova, and E. A. Sandykova "Improvement of the multiphoton fluorescence microscopy images quality using digital filtration", Proc. SPIE 10614, International Conference on Atomic and Molecular Pulsed Lasers XIII, 106141Q (16 April 2018); https://doi.org/10.1117/12.2303643

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