High Speed Striation Pattern Recognition In Contracting Cardiac Myocytes

Kenneth P. Roos; A. Christyne Bliton; Bradford A. Lubell; John M. Parker; Mark J. Patton; Stuart R. Taylor

doi:10.1117/12.951892

13 June 1989 High Speed Striation Pattern Recognition In Contracting Cardiac Myocytes

Kenneth P. Roos, A. Christyne Bliton, Bradford A. Lubell, John M. Parker, Mark J. Patton, Stuart R. Taylor

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951892
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

The understanding of muscle contraction and relaxation requires the quantitation of movement at the sub-micron level in living cells. Two complementary non-RS-170 imaging systems used for authentic real time measurement of contractile dynamics are described and compared. Images from isolated skeletal or cardiac muscle cells are projected by an optical microscope onto single line or area charge-coupled device (CCD) photodiode arrays. These data are digitized and stored for subsequent image processing and analysis. The inherently low contrast muscle striation patterns are enhanced and their rapid movement measured with an accuracy at least an order of magnitude greater than traditional limits of optical resolution. The features of each image format are complementary and when combined provide the maximum overall information in time and space.

Citation Download Citation

Kenneth P. Roos, A. Christyne Bliton, Bradford A. Lubell, John M. Parker, Mark J. Patton, and Stuart R. Taylor "High Speed Striation Pattern Recognition In Contracting Cardiac Myocytes", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951892

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