CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10662 > Article
Presentation
15 May 2018 Label-free bacteria detection using controllable microporous polyelectrolyte coated long-period fiber gratings (Conference Presentation)
Fan Yang, Tzu-Lan Chang, Henry Du, Junfeng Liang, Fei Tian
Author Affiliations +
Proceedings Volume 10662, Smart Biomedical and Physiological Sensor Technology XV; 106620J (2018) https://doi.org/10.1117/12.2304843
Event: SPIE Commercial + Scientific Sensing and Imaging, 2018, Orlando, FL, United States
Abstract
A novel application of a highly sensitive biosensor based on long-period fiber gratings (LPFG) coated with microporous polyelectrolyte coating for Gram-negative bacteria detection was investigated. The uniform microporous coating with large surface area was fabricated with weak polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) via layer-by-layer (LbL) assembly, followed by a brief exposure to acidic aqueous solutions at pH=4. The diameter of the micropores could be adjusted in a wide range by thermal treatment and ionic etching. The effect of pore size and surface topography on bacteria adhesion was examinged. Optofluidic LPFG platform for real-time monitoring of the bacteria binding/ adhesion in a flowing condition was investigated by measuring the spectral shift in the resonance wavelength. The coated LPFG platform was further functionalized with covalently immobilized bacteria antibody for specific bacterial detection with a concentration as low as 104 CFU/ml. Comparing with the widely used biosensors based on surface plasmon resonance (SPR), no moving part or metal deposition is required in our biosensor, making it highly sensitive, accurate, compact and cost effective.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Fan Yang, Tzu-Lan Chang, Henry Du, Junfeng Liang, and Fei Tian "Label-free bacteria detection using controllable microporous polyelectrolyte coated long-period fiber gratings (Conference Presentation)", Proc. SPIE 10662, Smart Biomedical and Physiological Sensor Technology XV, 106620J (15 May 2018); https://doi.org/10.1117/12.2304843
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Bacteria
Fiber coatings
Biosensors
Coating
Etching
Metals
Optofluidics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top