Quantifying the concentration of glucose, urea, and lactic acid in mixture by confocal Raman microscopy

Zhengyuan Tang; Sinead J. Barton; Tomas Ward; John Lowry; Michelle Doran; Hugh J. Byrne; Bryan M. Hennelly

doi:10.1117/12.2307550

17 May 2018 Quantifying the concentration of glucose, urea, and lactic acid in mixture by confocal Raman microscopy

Zhengyuan Tang, Sinead J. Barton, Tomas Ward, John Lowry, Michelle Doran, Hugh J. Byrne, Bryan M. Hennelly

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106850V (2018) https://doi.org/10.1117/12.2307550
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Raman spectroscopy has numerous applications in the field of biology. One such application is the simultaneously measurement of the concentration of multiple biochemical components in low volume aqueous mixtures, for example, a single drop of blood serum. Over twenty years ago, it was shown for the first time that it was possible to estimate the concentration of glucose, urea, and lactic acid in mixture by combining Raman Spectroscopy with Partial Least Squares Regression analysis. This was followed by numerous contributions in the literature designed to increase the number of components and reduce the limits of concentration that could be simultaneously measured using Raman spectroscopy, by developing various optical architectures to maximise the signal to noise ratio. The aim of this paper is to demonstrate the potential of a confocal Raman microscopy system for multicomponent analysis for the case of physiologically relevant mixtures of glucose, urea, and lactic acid.

Citation Download Citation

Zhengyuan Tang, Sinead J. Barton, Tomas Ward, John Lowry, Michelle Doran, Hugh J. Byrne, and Bryan M. Hennelly "Quantifying the concentration of glucose, urea, and lactic acid in mixture by confocal Raman microscopy", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106850V (17 May 2018); https://doi.org/10.1117/12.2307550

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