Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy

Takuya Sakaue; Eiji Hase; Takeo Minamikawa; Takeshi Yasui

doi:10.1117/12.2319273

24 April 2018 Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy

Takuya Sakaue, Eiji Hase, Takeo Minamikawa, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111D (2018) https://doi.org/10.1117/12.2319273
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Collagen plays an important role as a structural protein to determine the morphology and the mechanical property of tissues. Collagen orientation is one important parameter closely related with the mechanical property because collagen possesses fiber structure in tissues. However, it is difficult to evaluate the collagen fiber orientation quantitatively without invasiveness. In this article, we constructed continuously-polarization-resolved second-harmonic-generation (SHG) microscopy based on rapid polarization rotation every 15 degrees with electric-optic modulator, and applied it for the quantitative analysis of collagen fiber orientation. The orientation angle and degree of collagen fiber in different biological tissues were extracted from the curve fitting with a model function to a series of polarization-resolved

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Takuya Sakaue, Eiji Hase, Takeo Minamikawa, and Takeshi Yasui "Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111D (24 April 2018); https://doi.org/10.1117/12.2319273

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