Opto electronic tweezers based smart sweeper for cells/micro-particles sorting

R. S. Verma; N. Kumar

doi:10.1117/12.2317744

26 April 2018 Opto electronic tweezers based smart sweeper for cells/micro-particles sorting

R. S. Verma, N. Kumar

Proceedings Volume 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX; 1071613 (2018) https://doi.org/10.1117/12.2317744
Event: Saratov Fall Meeting 2017, 2017, Saratov, Russian Federation

Abstract

We report on use of opto-electronic tweezers based sorting approach, termed as smart sweepers, for sorting the microscopic particles by using the Dielectrophoretic (DEP) force response of cells on applied a.c. bias frequency. The applied a.c. bias was kept in negative DEP region, close to the crossover frequency of one of the particles. A line shaped intensity pattern, generated by a cylindrical lens, was scanned across the mixture sample. The particles whose cross over frequency was close to the applied bias frequency, experienced negligible negative DEP(n-DEP) force. On the other hand, the other type of particle experienced large repelling force and were forced to move along the scanning direction of the line shaped intensity profile. We, as a proof of concept, demonstrated the working principle of opto electronic smart sweepers by sweeping out the polystyrene particles from a mixture consisting of polystyrene microspheres (PSM) and red blood cells (RBCs) and leaving RBCs in the region of interest.

Citation Download Citation

R. S. Verma and N. Kumar "Opto electronic tweezers based smart sweeper for cells/micro-particles sorting", Proc. SPIE 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX, 1071613 (26 April 2018); https://doi.org/10.1117/12.2317744

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