Development of molecular probes for cellular imaging combining second harmonic generation and two-photon fluorescence

K. Clays

doi:10.1117/12.2323412

14 September 2018 Development of molecular probes for cellular imaging combining second harmonic generation and two-photon fluorescence

K. Clays

Author Affiliations +

Proceedings Volume 10738, Organic and Hybrid Sensors and Bioelectronics XI; 107381D (2018) https://doi.org/10.1117/12.2323412
Event: SPIE Organic Photonics + Electronics, 2018, San Diego, California, United States

Abstract

We review the development of molecular, exogenic probes for the application of nonlinear optical imaging. The emphasis necessarily is on requirements for second-harmonic imaging, because of the more stringent noncentrosymmetry requirements imposed at both the molecular and the structural level. We focus on the application in cellular imaging, where the challenge is the specificity. Because of the serendipitous use of charges on the probes for the optimization of both the amphiphilic and the optical properties, we recall how hyper-Rayleigh scattering has been instrumental in this development. This also holds true for the fluorescent proteins and examples of fluorescent and chromoproteins for nonlinear imaging will be presented.

Conference Presentation

Citation Download Citation

K. Clays "Development of molecular probes for cellular imaging combining second harmonic generation and two-photon fluorescence", Proc. SPIE 10738, Organic and Hybrid Sensors and Bioelectronics XI, 107381D (14 September 2018); https://doi.org/10.1117/12.2323412

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