Optogenetic control of cytokinesis shows that polarized trafficking governs the process

Jean A. Castillo-Badillo; Xenia Meshik; Suyash Harlalka; N. Gautam

doi:10.1117/12.2508792

22 February 2019 Optogenetic control of cytokinesis shows that polarized trafficking governs the process

Jean A. Castillo-Badillo, Xenia Meshik, Suyash Harlalka, N. Gautam

Proceedings Volume 10866, Optogenetics and Optical Manipulation 2019; 1086609 (2019) https://doi.org/10.1117/12.2508792
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optogenetics allows for the study of signaling pathways during a variety of cellular processes. Here we use subcellular optogenetics to study the role of vesicular trafficking in cytokinesis. Optogenetically activating RhoA in the cell midline induces cytokinesis and intercellular bridge formation. RhoA activates the actomyosin contractility network, leading to retrograde plasma membrane flow, localized decrease in membrane tension, and increase in endocytosis at the cell middle. Endocytic vesicles and trafficking proteins localize to the intercellular bridge. Perturbation of these proteins inhibits furrow formation. Thus polarized endocytosis and exocytosis in the first steps of cytokinesis provide new membrane to the middle of the cell and allow the cell to build the cleavage furrow and intercellular bridge.

Conference Presentation

Citation Download Citation

Jean A. Castillo-Badillo, Xenia Meshik, Suyash Harlalka, and N. Gautam "Optogenetic control of cytokinesis shows that polarized trafficking governs the process", Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 1086609 (22 February 2019); https://doi.org/10.1117/12.2508792

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available