The use of coated glass substrates for signal enhancement in multiphoton imaging

Sheng-Lin Lee; Han-Wen Guo; Yang-Fang Chen; Chen-Yuan Dong

doi:10.1117/12.2507182

4 March 2019 The use of coated glass substrates for signal enhancement in multiphoton imaging

Sheng-Lin Lee, Han-Wen Guo, Yang-Fang Chen, Chen-Yuan Dong

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108810T (2019) https://doi.org/10.1117/12.2507182
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In nonlinear optical imaging of biological samples, epi-illumination collection of fluorescent signals results in half of the signal loss. We enhanced the collected luminescent signal by using a multilayer coating of tantalum pentoxide (Ta₂O₅) and cerium oxide (SiO₂). Our coatings are biocompatible, allowing visual inspection of samples and optimizing the collection of luminescent signals. This method was confirmed on a number of samples, including sulforhodamine solutions, fluorescent microspheres and labeled 3T3 cells. In all cases, the coated coverslips were used to enhance the signal by approximately 2 times. Image analysis also shows that signal enhancement does not result in additional photobleaching. Our results indicate that the designed coated coverslip enhances multi-photon microscopy detection signals.

Citation Download Citation

Sheng-Lin Lee, Han-Wen Guo, Yang-Fang Chen, and Chen-Yuan Dong "The use of coated glass substrates for signal enhancement in multiphoton imaging", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810T (4 March 2019); https://doi.org/10.1117/12.2507182

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Glasses

Luminescence

Signal detection

Coating

Microscopes

Multiphoton microscopy

Tantalum

Show All Keywords

Keywords/Phrases

Search In:

Publication Years