Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella

Sheng-Lin Lee; Yang-Fang Chen; Chen-Yuan Dong

doi:10.1117/12.2507175

22 February 2019 Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella

Sheng-Lin Lee, Yang-Fang Chen, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 1088212 (2019) https://doi.org/10.1117/12.2507175
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Cornea is one of the collagen-rich connective tissues and plays an important role in vision. While X-ray scattering techniques are able to determine bulk structure of cornea, second harmonic generation microscopy can reveal depthdependent details of the corneal stroma comprised of a layered network of fibrillar collagen. In this work, we used Fast Fourier Transform second harmonic generation microscopy as a tool to determine the directionality of corneal stroma as a function of depth. Our results also display the position dependent difference of corneal stroma architecture.

Conference Presentation

Citation Download Citation

Sheng-Lin Lee, Yang-Fang Chen, and Chen-Yuan Dong "Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088212 (22 February 2019); https://doi.org/10.1117/12.2507175

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available