Phasor plot analysis using low pass filter for high-speed FLIM

Dongeun Kim; Wonsang Hwang; Youngjae Won; Sucbei Moon; Sang Yoon Lee; Mingu Kang; Won Sub Han; Dug Young Kim

doi:10.1117/12.2509325

22 February 2019 Phasor plot analysis using low pass filter for high-speed FLIM

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Mingu Kang, Won Sub Han, Dug Young Kim

Proceedings Volume 10884, Single Molecule Spectroscopy and Superresolution Imaging XII; 108840P (2019) https://doi.org/10.1117/12.2509325
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

Conference Presentation

Citation Download Citation

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Mingu Kang, Won Sub Han, and Dug Young Kim "Phasor plot analysis using low pass filter for high-speed FLIM", Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840P (22 February 2019); https://doi.org/10.1117/12.2509325

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