Portable bioluminescence detection for food safety: smartphone vs. silicon photomultiplier

Iyll-Joon Doh; Hyun Jung Min; Claudia Coronel-Aguilera; Trevor Lim; Bruce M. Applegate; Euiwon Bae

doi:10.1117/12.2521452

30 April 2019 Portable bioluminescence detection for food safety: smartphone vs. silicon photomultiplier

Iyll-Joon Doh, Hyun Jung Min, Claudia Coronel-Aguilera, Trevor Lim, Bruce M. Applegate, Euiwon Bae

Proceedings Volume 11016, Sensing for Agriculture and Food Quality and Safety XI; 110160E (2019) https://doi.org/10.1117/12.2521452
Event: SPIE Defense + Commercial Sensing, 2019, Baltimore, MD, United States

Abstract

Luminescence based detection has been widely used in diverse science and engineering applications. The recent development of the smartphone has enabled end users to utilize this communication device as a portable detector and instruments such as a microscope, fluorimeter, colorimeter, and spectrometer. To transform the smartphone into a bioluminescence detector, our group developed an advanced signal processing algorithm and an optical chamber designed for efficient photon capture. This solution was required to overcome the typical sensitivity of the CMOS-based smartphone camera such that sub-nano to pico Watt levels of power can be measured with conventional smartphones. Preliminary experiments conducted with the bioluminescent Pseudomonas fluorescens M3A shows a detection limit of approximately 10⁶ CFU/ml. To achieve sensitive detection while maintaining the portability, we explored using the recently developed silicon photomultiplier (SiPM), and designed a portable bioluminescence sensor which shows a 2-3 order higher sensitivity on calibration sample testing. Finally, for live sample testing, Escherichia coli O157:H7 was inoculated on a ground beef sample and subjected to luminescence phage based detection and a luminescence signal was generated from the bacteriophage infection and detected within 8-10 h after spiking.

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Iyll-Joon Doh, Hyun Jung Min, Claudia Coronel-Aguilera, Trevor Lim, Bruce M. Applegate, and Euiwon Bae "Portable bioluminescence detection for food safety: smartphone vs. silicon photomultiplier", Proc. SPIE 11016, Sensing for Agriculture and Food Quality and Safety XI, 110160E (30 April 2019); https://doi.org/10.1117/12.2521452

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KEYWORDS

Bioluminescence

Sensors

Signal to noise ratio

Luminescence

Silicon photomultipliers

Signal detection

Algorithm development

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