Results on identification of bacteria aging in complex environmental samples using Raman spectroscopy

V. Rebuffel; J.-C. Baritaux; E. Bergmann; P. Marcoux; I. Espagnon; S. Morales

doi:10.1117/12.2527141

22 July 2019 Results on identification of bacteria aging in complex environmental samples using Raman spectroscopy

V. Rebuffel, J.-C. Baritaux, E. Bergmann, P. Marcoux, I. Espagnon, S. Morales

Proceedings Volume 11075, Novel Biophotonics Techniques and Applications V; 110750J (2019) https://doi.org/10.1117/12.2527141
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Spontaneous Raman scattering is a reliable technique for fast identification of single bacterial cells, when spectra are acquired in laboratory conditions where bacteria growth and state are controlled. We have developed a multi-modal system combining Raman spectroscopy and darkfield imaging, aiming at analysing environmental samples, typically in the field context of biological pathogens detection. Such samples are heterogeneous, both in terms of phenotype content and environmental matrix, even after a preliminary purification step. In this paper, we report a study on the identification of Bacillus Thuriengensis (BT) mimicing pathogen bacteria, embedded in a real-world matrix: a sample of surface water enriched with environmental bacterial species. The purpose is to evaluate both the detection limit of aging BT over time and the false alarm rate, in the conditions of our experiment.

Citation Download Citation

V. Rebuffel, J.-C. Baritaux, E. Bergmann, P. Marcoux, I. Espagnon, and S. Morales "Results on identification of bacteria aging in complex environmental samples using Raman spectroscopy ", Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750J (22 July 2019); https://doi.org/10.1117/12.2527141

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