High accuracy platelet counting using lensfree imaging

D. Isèbe; E. Gremion; A. Daynès; B. Thouy; N. Rongeat; S. Bressieux; A. Ali-Cherif; V. Rebuffel; P. Joly; S. Morales; O. Cioni; P. Blandin

doi:10.1117/12.2527008

22 July 2019 High accuracy platelet counting using lensfree imaging

D. Isèbe, E. Gremion, A. Daynès, B. Thouy, N. Rongeat, S. Bressieux, A. Ali-Cherif, V. Rebuffel, P. Joly, S. Morales, O. Cioni, P. Blandin

Author Affiliations +

Proceedings Volume 11075, Novel Biophotonics Techniques and Applications V; 110750O (2019) https://doi.org/10.1117/12.2527008
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Flow cytometry is the main technology used in hematology analyzers. However, this technology requires bulky and complex hardware systems. Lens-free imaging is an emerging microscopy technique based on a simple and compact inline holography setup. This technique enables to image a large field-of-view (≈30mm²) leading to statistical counting (>10 000 cells) in a single-shot acquisition consistent with performances required in hematology. We report high accuracy platelet count in 54 platelet-rich plasma samples. This accuracy can be achieved through a wise choice of the illumination spectral properties and an optimized algorithmic chain dedicated to small pure phase objects.

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D. Isèbe, E. Gremion, A. Daynès, B. Thouy, N. Rongeat, S. Bressieux, A. Ali-Cherif, V. Rebuffel, P. Joly, S. Morales, O. Cioni, and P. Blandin "High accuracy platelet counting using lensfree imaging", Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750O (22 July 2019); https://doi.org/10.1117/12.2527008

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