Synergistic effects of combination of three wavelengths and different light sources in cytochrome c oxidase activity in intact skeletal muscle of rats

Ernesto Cesar Pinto Leal-Junior; Shaiane da Silva Tomazoni

doi:10.1117/12.2526834

22 July 2019 Synergistic effects of combination of three wavelengths and different light sources in cytochrome c oxidase activity in intact skeletal muscle of rats

Ernesto Cesar Pinto Leal-Junior, Shaiane da Silva Tomazoni

Author Affiliations +

Proceedings Volume 11079, Medical Laser Applications and Laser-Tissue Interactions IX; 110791E (2019) https://doi.org/10.1117/12.2526834
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

This study aimed to investigate the effects of PBMT with a synergistic combination of lasers and light emitting diodes (LEDs) with three different wavelengths and three doses on cytochrome c oxidase activity in intact skeletal muscle, and also to determine the time-window between irradiation and increased activity on cytochrome c-oxidase in skeletal muscle fibers. Male Wistar rats (240 ± 20g) were divided into two groups (placebo and PBMT) where seven different experimental time-points were tested. Transcutaneous PBMT was irradiated at tibialis anterior muscles employing a cluster with 9 diodes (1 laser diode of 905nm, 4 LED diodes of 875nm, 4 LED diodes of 640nm – manufactured by Multi Radiance Medical™), with different doses (1, 3 and 10 J). Then muscles were removed at different experimental time-points (5, 10, 30 minutes, 1, 2, 12 and 24 hours) and the analyses of cytochrome c oxidase expression were performed by immunohistochemistry. PBMT with 10 J dose increased cytochrome c oxidase expression with significant difference (p<0.05) when compared to the placebo group at all time-points tested. The 3 J dose showed significant differences (p<0.05) when compared to the placebo group from 30 mins to 12 h time-points, and the 1J dose showed significant differences (p<0.05) when compared to the placebo group from 30 mins to 24 h. PBMT with the combination of super-pulsed laser, red and infrared LEDs can increase cytochrome c-oxidase activity in intact skeletal muscle mainly with 10 J dose fulfilling the time-response window from 5 minutes until 24 hours after irradiation.

Citation Download Citation

Ernesto Cesar Pinto Leal-Junior and Shaiane da Silva Tomazoni "Synergistic effects of combination of three wavelengths and different light sources in cytochrome c oxidase activity in intact skeletal muscle of rats", Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110791E (22 July 2019); https://doi.org/10.1117/12.2526834

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Light emitting diodes

Diodes

Statistical analysis

Light sources

Laser therapeutics

Semiconductor lasers

Infrared lasers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years