Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings

Anna-Lena Robisch; Marina Eckermann; Mareike Töpperwien; Franziska van der Meer; Christine Stadelmann; Tim Salditt

doi:10.1117/12.2529041

10 September 2019 Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings

Anna-Lena Robisch, Marina Eckermann, Mareike Töpperwien, Franziska van der Meer, Christine Stadelmann, Tim Salditt

Author Affiliations +

Proceedings Volume 11113, Developments in X-Ray Tomography XII; 1111304 (2019) https://doi.org/10.1117/12.2529041
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

X-ray cone-beam holo-tomography of unstained tissue from the human central nervous system reveals details down to sub-cellular length scales.¹ This visualization of variations in the electron density of the sample is based on phase contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength independent electron density. In this contribution we present multi-E holo-tomography at the GINIX setup of the P10 beamline at DESY. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial Filtering and high numerical aperture.² Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue.¹ We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a double crystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed.

Citation Download Citation

Anna-Lena Robisch, Marina Eckermann, Mareike Töpperwien, Franziska van der Meer, Christine Stadelmann, and Tim Salditt "Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings", Proc. SPIE 11113, Developments in X-Ray Tomography XII, 1111304 (10 September 2019); https://doi.org/10.1117/12.2529041

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available