Microscopic urinalysis by digital holographic microscopy

Vivek Rastogi; Shilpi Agarwal; Satish Kumar Dubey; Gufran Sayeed Khan; Chandra Shakher

doi:10.1117/12.2537315

18 November 2019 Microscopic urinalysis by digital holographic microscopy

Vivek Rastogi, Shilpi Agarwal, Satish Kumar Dubey, Gufran Sayeed Khan, Chandra Shakher

Proceedings Volume 11188, Holography, Diffractive Optics, and Applications IX; 1118818 (2019) https://doi.org/10.1117/12.2537315
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Urine analysis (urinalysis) is a critical component to diagnose urinary tract disease. Microscopic evaluation of the urine provides an insight into potential underlying urinary tract disease, which is used for identification and characterization of both common and much less common formed elements in the urine sample. In this paper, the microscopic urinalysis is presented by using single beam digital holographic microscopy (DHM). This is a common path set up wherein both beams (reference and sample) travel through a similar path providing higher temporal stability. In this paper, phasecontrast three - dimensional imaging of red blood cells (erythrocytes), white blood cells (leukocytes), squamous and nonsquamous epithelial cells, casts and various crystals present in the urine samples, have been demonstrated. The proposed imaging modality for the diagnosis of urinary tract disease is simple, non-contact, non-invasive, and provides higher temporal stability due to its common-path geometry.

Citation Download Citation

Vivek Rastogi, Shilpi Agarwal, Satish Kumar Dubey, Gufran Sayeed Khan, and Chandra Shakher "Microscopic urinalysis by digital holographic microscopy", Proc. SPIE 11188, Holography, Diffractive Optics, and Applications IX, 1118818 (18 November 2019); https://doi.org/10.1117/12.2537315

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