FPGA-based real-time signal triggered storage system for ultrafast imaging flow cytometry

Honghao Huang; Weizhe Meng; Wanyue Zhao; Minghua Chen; Sigang Yang; Hongwei Chen

doi:10.1117/12.2536524

20 November 2019 FPGA-based real-time signal triggered storage system for ultrafast imaging flow cytometry

Honghao Huang, Weizhe Meng, Wanyue Zhao, Minghua Chen, Sigang Yang, Hongwei Chen

Proceedings Volume 11192, Real-time Photonic Measurements, Data Management, and Processing IV; 111920T (2019) https://doi.org/10.1117/12.2536524
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Ultrafast imaging flow cytometry can be realized by time-encoded single-pixel imaging technique, with high imaging speed (<10million frame/s) and high throughput (<10,000 cells/s). However, the signal of background image without cells occupies a large part of the acquired data and takes up a lot of storage space. In this paper, a FPGA-based triggering and storage system is proposed, which allows real-time storage of signal of cells with blank background neglected. Moreover, it is easy to implement and of high accuracy, as well as adaptivity to different sampling rate. This system reduces the required storage space and enables efficient storage for ultrafast imaging flow cytometry.

Citation Download Citation

Honghao Huang, Weizhe Meng, Wanyue Zhao, Minghua Chen, Sigang Yang, and Hongwei Chen "FPGA-based real-time signal triggered storage system for ultrafast imaging flow cytometry", Proc. SPIE 11192, Real-time Photonic Measurements, Data Management, and Processing IV, 111920T (20 November 2019); https://doi.org/10.1117/12.2536524

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