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9 March 2020 In vivo validation of OCT hemodynamic measurement techniques with two-photon microscopy (Conference Presentation)
Paul J. Marchand, Xuecong Lu, Cong Zhang, Yuankang Lu, Frédéric Lesage
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Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 112280Y (2020) https://doi.org/10.1117/12.2546720
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Over the past decades, several techniques have been developed to measure cerebral hemodynamic parameters such as red blood cell velocity, flux and hematocrit using optical coherence tomography (OCT). Despite these advancements, two photon microscopy (TPM) remains the gold standard for functional cerebral hemodynamic imaging, though localized line-scan or bolus tracking. Here we developed an optical platform enabling concurrent imaging with OCT and TPM and perform co-localized and simultaneous velocimetric measurements with both modalities, with the aim of validating OCT velocimetric methods.
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Paul J. Marchand, Xuecong Lu, Cong Zhang, Yuankang Lu, and Frédéric Lesage "In vivo validation of OCT hemodynamic measurement techniques with two-photon microscopy (Conference Presentation)", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112280Y (9 March 2020); https://doi.org/10.1117/12.2546720
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KEYWORDS
Optical coherence tomography
Hemodynamics
Two photon excitation microscopy
In vivo imaging
3D acquisition
3D image processing
Blood
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