Compressed hyperspectral Raman microscope for imaging tissues and cellular structures

Mark A. Keppler; Eddie M. Gil; Sean P. O'Connor; Gary Noojin; Vladislav V. Yakovlev; Joel N. Bixler

doi:10.1117/12.2544571

20 February 2020 Compressed hyperspectral Raman microscope for imaging tissues and cellular structures

Mark A. Keppler, Eddie M. Gil, Sean P. O'Connor, Gary Noojin, Vladislav V. Yakovlev, Joel N. Bixler

Proceedings Volume 11238, Optical Interactions with Tissue and Cells XXXI; 112380N (2020) https://doi.org/10.1117/12.2544571
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Raman imaging continues to grow in popularity as a label-free technique for characterizing the underlying chemical structure of biological materials, both in-vitro and in-vivo. While Raman spectra demonstrate high chemical specificity, spontaneous Raman scattering is an inherently weak process and requires prohibitively long acquisition times. When Raman is utilized to image highly scattering cellular environments, integration times can be on the order of several minutes to hours. Recently developed compressed sensing techniques can greatly improve hyperspectral Raman acquisition times by randomly under-sampling the spatial dimensions. A digital micromirror device (DMD) is used to spatially encode the image plane. The encoded image is then propagated to a spectrometer where the spectral components are produced by shearing one spatial dimension. Several reconstruction algorithms have been developed that can then be used to return the original. Here, we will present single-shot, 2D Raman imaging of CHO cells using compressed hyperspectral Raman microscope. This system provides an order of magnitude improvement on traditional hyperspectral acquisition rates. Single-shot compressed hyperspectral Raman images can reveal biochemical changes due to short lifetime dynamic processes. These improvements will allow imaging of samples that metabolize quickly, rapidly oxidize, or are physically altered under experimental conditions.

Citation Download Citation

Mark A. Keppler, Eddie M. Gil, Sean P. O'Connor, Gary Noojin, Vladislav V. Yakovlev, and Joel N. Bixler "Compressed hyperspectral Raman microscope for imaging tissues and cellular structures", Proc. SPIE 11238, Optical Interactions with Tissue and Cells XXXI, 112380N (20 February 2020); https://doi.org/10.1117/12.2544571

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