MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy (Conference Presentation)

Francisco Balzarotti; Jasmin K. Pape; Klaus C. Gwosch; Stefan W Hell

doi:10.1117/12.2553661

9 March 2020 MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy (Conference Presentation)

Francisco Balzarotti, Jasmin K. Pape, Klaus C. Gwosch, Stefan W Hell

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460S (2020) https://doi.org/10.1117/12.2553661
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Superresolution microscopy methods have revolutionized far-field optical fluorescence microscopy by manipulating state transitions of the emitters, offering potentially unlimited resolution. In practice, however, the resolution of an image is limited by the finite photon budget of fluorescent probes. The recently introduced localization concept, termed MINFLUX, tackles this limitation by rendering each emitted photon more informative, achieving single digit nanometer resolution. Here, we present a MINFLUX strategy with high photon efficiency in arbitrarily large regions that allows imaging in fixed and living cells. This allows isotropic localization precision and surpasses the typical ∝1⁄√N dependence. A multi-color modality for 3D-MINFLUX imaging will be also presented, together with several biological applications.

Conference Presentation

Citation Download Citation

Francisco Balzarotti, Jasmin K. Pape, Klaus C. Gwosch, and Stefan W Hell "MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460S (9 March 2020); https://doi.org/10.1117/12.2553661

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