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10 March 2020 Spatial frequency projection imaging of coherent anti-Stokes Raman scattering (SPIFI-CARS) (Conference Presentation)
Sandro Heuke, Siddharth Sivankutty, Camille Scotte, Patrick A. Stockton, Randy A. Bartels, Anne Sentenac, Hervé Rigneault
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Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125203 (2020) https://doi.org/10.1117/12.2546002
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Conventional CARS microscopy requires scanning a point focus through the specimen limits imaging speed. We present a spatial frequency projection imaging (SPIFI) method for CARS microscopy to spatially multiplex CARS microscopy. A spinning disk modulator is used to rapidly modulate the Stokes field with a rapidly swept spatially periodic transmission grating. SPIFI-CARS images are obtained by Fourier transforming the single pixel signal. Images of CARS and second harmonic generation from histological slices will be presented. The physics of image formation and the impact of multiplexing on SNR will be discussed. Prospects for scaling to high speed CARS imaging will be discussed.
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Sandro Heuke, Siddharth Sivankutty, Camille Scotte, Patrick A. Stockton, Randy A. Bartels, Anne Sentenac, and Hervé Rigneault "Spatial frequency projection imaging of coherent anti-Stokes Raman scattering (SPIFI-CARS) (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125203 (10 March 2020); https://doi.org/10.1117/12.2546002
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KEYWORDS
Coherence imaging
CARS tomography
Spatial frequencies
Image processing
Microscopy
Process modeling
Imaging spectroscopy
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