A single particle sensing platform based on 2D gold nanocrystals coated with designable artificial repeat proteins (Conference Presentation)

Laureen Moreaud; Janak Prasad; Sirin Celiksoy; Sébastien Voillet; Agathe Urvoas; Marie Valerio-Lepiniec; Philippe Minard; Carsten Soennichsen; Erik Dujardin

doi:10.1117/12.2544284

10 March 2020 A single particle sensing platform based on 2D gold nanocrystals coated with designable artificial repeat proteins (Conference Presentation)

Laureen Moreaud, Janak Prasad, Sirin Celiksoy, Sébastien Voillet, Agathe Urvoas, Marie Valerio-Lepiniec, Philippe Minard, Carsten Soennichsen, Erik Dujardin

Author Affiliations +

Proceedings Volume 11255, Colloidal Nanoparticles for Biomedical Applications XV; 112550D (2020) https://doi.org/10.1117/12.2544284
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Shape control of metal nanocrystals with engineered proteins is promising for integrated plasmonics, biosensing, and catalysis applications. We present artificial thermo-stable proteins used as inhibitors during gold nanocrystal growth in order to control the shape and crystallographic facet expression. This synthesis leads to (111)-terminated gold nanocrystals in high yield. The shape and size distribution are tuned towards nanoplates by adjusting synthetic conditions. When biotinylated alpha-Reps are used, the morphosynthetic properties are preserved and streptavidin sensing is observed through a marked 7-nm redshift in dark-field spectra. Single nanoplate streptavidin sensing experiments show high sensitivity (300 nm/RIU).

Conference Presentation

Citation Download Citation

Laureen Moreaud, Janak Prasad, Sirin Celiksoy, Sébastien Voillet, Agathe Urvoas, Marie Valerio-Lepiniec, Philippe Minard, Carsten Soennichsen, and Erik Dujardin "A single particle sensing platform based on 2D gold nanocrystals coated with designable artificial repeat proteins (Conference Presentation)", Proc. SPIE 11255, Colloidal Nanoparticles for Biomedical Applications XV, 112550D (10 March 2020); https://doi.org/10.1117/12.2544284

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