Modelling the wavelength-dependent response of SHG excitation efficiency

Lichuan Li; Stephen J. Matcher

doi:10.1117/12.2555687

1 April 2020 Modelling the wavelength-dependent response of SHG excitation efficiency

Lichuan Li, Stephen J. Matcher

Proceedings Volume 11359, Biomedical Spectroscopy, Microscopy, and Imaging; 1135909 (2020) https://doi.org/10.1117/12.2555687
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

As a non-invasive imaging tool, second harmonic generation (SHG) imaging has shown a great prospect in the visualization of living cells and tissues. It is widely used in the fields of science, medicine, biology and tissue engineering. Although the SHG intensity is often used as the basis of imaging analysis, its interpretation is still very subjective. In this project, the nonlinear process behind SHG is described. In order to explore the wavelength-dependence of SHG, the simulation experiments are carried out. A contrast model is used to calculate the forward- and backward- generated SHG signals. The simulation results show the SHG intensities of different collagen models at the wavelength between 760 nm to 1000 nm.

Conference Presentation

Citation Download Citation

Lichuan Li and Stephen J. Matcher "Modelling the wavelength-dependent response of SHG excitation efficiency", Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 1135909 (1 April 2020); https://doi.org/10.1117/12.2555687

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