Digital holography for the immersive live exploration and manipulation of 3D micro-systems

Stefano Ferretti; Silvio Bianchi; Giacomo Frangipane; Roberto Di Leonardo

doi:10.1117/12.2570817

23 August 2020 Digital holography for the immersive live exploration and manipulation of 3D micro-systems

Stefano Ferretti, Silvio Bianchi, Giacomo Frangipane, Roberto Di Leonardo

Proceedings Volume 11463, Optical Trapping and Optical Micromanipulation XVII; 114630F (2020) https://doi.org/10.1117/12.2570817
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

Computational approaches to microscopy allow high frame volumetric imaging by numerical reconstructions from 2D holographic patterns that encode the full 3D structure of the scene. Furthermore, highly efficient iterative algorithms ensure quick hologram computation for the live SLM refresh in the optical trapping system. The combination of holographic optical tweezers and a 3-axis implementation of holographic microscopy in the same setup leads to simultaneous dynamical manipulation and real-time volume reconstruction of microscopic samples. We present a novel interface to holographic optical tweezers that allows for an immersive and interactive experience of micromanipulation of colloids and living cells.

Conference Presentation

Citation Download Citation

Stefano Ferretti, Silvio Bianchi, Giacomo Frangipane, and Roberto Di Leonardo "Digital holography for the immersive live exploration and manipulation of 3D micro-systems", Proc. SPIE 11463, Optical Trapping and Optical Micromanipulation XVII, 114630F (23 August 2020); https://doi.org/10.1117/12.2570817

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