Tip-Enhanced Raman Spectroscopy (TERS) for nanoscale imaging of biological membranes

Giovanni Luca Bartolomeo; Guillaume Goubert; Renato Zenobi

doi:10.1117/12.2568010

20 August 2020 Tip-Enhanced Raman Spectroscopy (TERS) for nanoscale imaging of biological membranes

Giovanni Luca Bartolomeo, Guillaume Goubert, Renato Zenobi

Proceedings Volume 11468, Enhanced Spectroscopies and Nanoimaging 2020; 114680S (2020) https://doi.org/10.1117/12.2568010
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

Tip-enhanced Raman spectroscopy (TERS) is a label-free imaging technique that combines scanning probe microscopy with Raman spectroscopy to obtain local chemical information, well below the optical diffraction limit. Discovered in the early 2000s, it has now become the tool-of-choice for the nanoscale investigation of carbon-based materials and 2D polymers. The perspective of imaging biological samples is attractive, but it is largely hampered by their low Raman cross-section and by their tendency to degrade quickly under the TERS tip. In this work, we propose to overcome these obstacles using stable tips as well as an effective sample deposition method to achieve nanoscale spatial and chemical characterization of protein/lipid membranes. As model samples, biomimetic membranes and bacteriorhodopsin (BR) membrane (from Halobacterium Salinarum) are tested.

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Giovanni Luca Bartolomeo, Guillaume Goubert, and Renato Zenobi "Tip-Enhanced Raman Spectroscopy (TERS) for nanoscale imaging of biological membranes", Proc. SPIE 11468, Enhanced Spectroscopies and Nanoimaging 2020, 114680S (20 August 2020); https://doi.org/10.1117/12.2568010

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KEYWORDS

Raman spectroscopy

Biological research

Statistical analysis

Catalysis

Diffraction

Imaging spectroscopy

Light-matter interactions

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