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5 March 2021 Kinetic analysis of lipid metabolism in live breast cancer cells via nonlinear optical microscopy
Nellone Reid, Jue Hou, Eric Potma, Bruce Tromberg
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Proceedings Volume 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII; 1162505 (2021) https://doi.org/10.1117/12.2577702
Event: SPIE BiOS, 2021, Online Only
Abstract
Combining coherent Raman scattering microscopy and deuterium labeling provides insight into metabolism in live cancer cells during cancer development and progression. The dynamic data is modeled with Michaelis-Menten-kinetics to quantify lipid synthesis and utilization in cancer cells. Changes in lipid levels are found to originate from de novo lipid synthesis using glucose as a source. In this work, we isolate fatty acid synthesis/consumption rates and elucidated effects of altered lipid metabolism in T47D breast cancer cells in response to estradiol stimulation and etomoxir treatment, dynamic processes that cannot be easily observed without the application of appropriate models.
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Nellone Reid, Jue Hou, Eric Potma, and Bruce Tromberg "Kinetic analysis of lipid metabolism in live breast cancer cells via nonlinear optical microscopy", Proc. SPIE 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII, 1162505 (5 March 2021); https://doi.org/10.1117/12.2577702
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KEYWORDS
Breast cancer
Microscopy
Mode conditioning cables
Optical microscopy
Cancer
Nondestructive evaluation
Raman scattering
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