A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging

Brook K. Byrd; Rendall S. Strawbridge; Wendy Wells; Connor Barth; Summer Gibbs; Scott C. Davis

doi:10.1117/12.2582542

5 March 2021 A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging

Brook K. Byrd, Rendall S. Strawbridge, Wendy Wells, Connor Barth, Summer Gibbs, Scott C. Davis

Author Affiliations +

Proceedings Volume 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII; 116250X (2021) https://doi.org/10.1117/12.2582542
Event: SPIE BiOS, 2021, Online Only

Abstract

Fluorescent contrast agents targeted to cancer biomarkers are increasingly being explored for cancer detection, surgical guidance, and response monitoring. Efforts have been underway to topically apply such biomarker-targeted agents to freshly excised specimen for detecting cancer cell receptors on the surface as a method for intraoperative surgical margin assessment, including dual-probe staining methods introduce a second ‘non-specific’ optical agent as a control to help compensate for heterogeneous uptake and normalize the imaging field. Still, such specimen staining protocols introduce multifaceted complexity with unknown variables, such as tissue-specific diffusion, cell-specific binding and disassociation rates, and other factors, affecting the interpreted cancer-biomarker distribution across the specimen surface. The ability to recover three-dimensional dual-probe biodistributions throughout whole-specimens could offer a ground-truth validation method for examining topical staining uptake behaviors. Herein, we report on a novel method for characterizing dual-probe accumulation with 3D depth-profiles observed from a dual-probe fresh-specimen staining experiment.

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Citation Download Citation

Brook K. Byrd, Rendall S. Strawbridge, Wendy Wells, Connor Barth, Summer Gibbs, and Scott C. Davis "A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging", Proc. SPIE 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII, 116250X (5 March 2021); https://doi.org/10.1117/12.2582542

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