The signal of optical coherence tomography (OCT) decays exponentially in depth due to tissue scattering, resulting in indistinct tissue features in three-dimension. Moreover, due to limited light penetration depth, extensive volumetric investigation is usually constrained for large-scale biological samples. By integrating serial sectioning technology with block-face imaging, we establish a volumetric OCT acquisition and reconstruction pipeline that incorporates depth-resolved attenuation coefficient estimation, volumetric stitching and filtering, and feature enhancement visualization. We demonstrate this pipeline on ex vivo human brain volumes of several cubic centimeters with 5 um isotropic resolution.
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