Wide-field imaging of brain white matter fiber tracts with Mueller polarimetry in backscattering configuration

Tatiana Novikova; Philippe Schucht; Hee Ryung Lee; Mohammed Hachem Mezouar; Ekkehard Hewer; Andreas Raabe; Michael Murek; Irena Zubak; Johannes Goldberg; Enikö Kövari; Angelo Pierangelo

doi:10.1117/12.2588957

5 March 2021 Wide-field imaging of brain white matter fiber tracts with Mueller polarimetry in backscattering configuration

Tatiana Novikova, Philippe Schucht, Hee Ryung Lee, Mohammed Hachem Mezouar, Ekkehard Hewer, Andreas Raabe, Michael Murek, Irena Zubak, Johannes Goldberg, Enikö Kövari, Angelo Pierangelo

Author Affiliations +

Proceedings Volume 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII; 1164105 (2021) https://doi.org/10.1117/12.2588957
Event: SPIE BiOS, 2021, Online Only

Abstract

The crucial problem of brain tumor surgery is the accurate detection the tumor border for safe and complete tumor resection. Whereas it is quite easy to identify brain tumor in preoperative magnetic resonance imaging, it is often difficult to differentiate solid tumor tissue from infiltrated white matter during surgery with conventional surgical intra-operative microscope. To address this problem we suggest exploring the optical anisotropy of healthy brain white matter which represents a highly ordered structure consisting of axons that are joined together in fiber tracts. Tumor cells grow chaotically and erase the optical anisotropy of healthy brain. Instead of detecting the tumor itself, we suggest to visualize healthy white matter by means of its fiber tracts by detecting the optical anisotropy of brain tissue. For this purpose we used a wide-field imaging Mueller polarimetric system operating in the visible wavelength range in backscattering configuration. The Mueller matrix images of the thick (~1cm) fixed human brain specimen and thick (~1cm) fresh veal brain specimen were measured at 633 nm in reflection. Lu Chipman decomposition was applied pixel-wise to the experimental Mueller matrices. The maps of azimuth of fast optical axis of linear birefringent medium showed a compelling correlation with the fiber tracts directions on histology image of thin whole mount silver-stained brain tissue section, that is gold standard for ex-vivo brain fiber tract visualization. Thus, label-free non-contact imaging Mueller polarimetry shows potential for the intra-operative visualization of brain white matter fiber tracts. Further studies are ongoing.

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Tatiana Novikova, Philippe Schucht, Hee Ryung Lee, Mohammed Hachem Mezouar, Ekkehard Hewer, Andreas Raabe, Michael Murek, Irena Zubak, Johannes Goldberg, Enikö Kövari, and Angelo Pierangelo "Wide-field imaging of brain white matter fiber tracts with Mueller polarimetry in backscattering configuration", Proc. SPIE 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII, 1164105 (5 March 2021); https://doi.org/10.1117/12.2588957

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